Differentiation studies: Multilineage differentiation potential of cultured BM-MSCs injected via different bore size needles along with controls. (A - D) Chondrogenic differentiation potential was demonstrated by Alcian blue staining. (E - H) Formation of mineralized matrix was detected by alizarin Red staining confirms the osteogenic differentiation. (I - L) Adipogenesis was confirmed by neutral oil droplet formation stained with Oil Red O. (M) Represents viability, stromal marker expression and mesodermal tri-lineage differentiation potential of MSCs ejected through 26 G needle after cryopreservation.