Figure 1

Binding of Surek and control antibodies. (A) Three different production batches A, B and C of Surek were compared for bispecific binding to either GD2 positive B78-D14 or to mouse CD3 expressing LBRM-33 cell lines by flow cytometry. In mean, binding to mouse CD3 (EC₅₀ = 0.16 μg/ml [1.07 nM]) was 23 times stronger than binding to GD2 (EC₅₀ = 3.6 μg/ml [24 nM]). (B) Binding curves of the monoclonal antibodies Me361, 14G2a, Surek and Ektomab to GD2 as measured by ELISA are displayed. Additionally, cross-reaction of Surek with GD3 is included. The figure shows one representative of three independent experiments. Mean calculated EC₅₀ values were 0.018 μg/ml [0.12 nM] (Me361), 0.073 μg/ml [0.49 nM] (14G2a), 4.8 μg/ml [32 nM] (Surek) and 3.1 μg/ml [20.7 nM] (Ektomab).