Figure 4
MMP9 expression and activity. A) MMP9 gene expression in HK2 cells treated with FGF-2 and sulodexide was measured by real-time PCR. Results represent the mean ± SD of two independent experiments performed in triplicate. B) Above, a representative gelatin zymography displaying MMP9 digestion bands produced by serum-free medium of HK2 cells cultured for 24 hours with FGF-2 and sulodexide. Below, densitometric analysis of MMP9 digestion bands expressed as a percentage of untreated cells. Results represent the mean ± SD of three independent experiments performed in triplicate.