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Figure 2 | Journal of Translational Medicine

Figure 2

From: Topoisomerase II alpha gene amplification is a favorable prognostic factor in patients with HER2-positive metastatic breast cancer treated with trastuzumab

Figure 2

Fluorescence in situ hybridization (FISH) in breast cancer cell lines and invasive breast carcinomas (IBC). FISH in breast cancer cell lines (A-F) and IBC cases (G-L) for the HER2 and TOP2A genes using the HER2/TOP2A/CEN17 triple color probe. The MDA-MB-231 cell line showed normal status of the HER2 and TOP2A genes (A-C), whereas in the SK-BR-3 cell line, co-amplification of the HER2 and TOP2A genes was found (D-F). IBC case showing simultaneous amplification of the HER2 and TOP2A genes (G-I); IBC case with amplification of the HER2 gene, deletion of the TOP2A gene and normal chromosome 17 status (J-L). Panels A, D, G and J show HER2 and centromere 17 hybridization signals; Panels B, E, H and K show TOP2A and centromere 17 hybridization signals, where as panels C, F, I and L depict merged HER2, TOP2A and centromere 17 signals. Magnification x1000.

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