Mcl-1 expression in neutrophils exposed to various oxygen conditions. (A) A representative western blot depicting Bax and Mcl-1 expression in normoxia (Nox), intermittent hypoxia (IH) and sustained hypoxia (SH) in one out of six experiments performed. (B) The normalized values of Mcl-1, obtained by densitometric analysis of western blots are presented as arbitrary units, and depicted as fold induction over β-actin in Nox, IH and SH. (C) Mcl-1 expression was assessed by confocal laser scanning microscopy (n=8). The average fluorescence intensity unit per cell, detected by immunofluorescence quantitation at normoxia was considered as baseline (100%), and the effects of IH and SH were calculated as relative Mcl-1increase. P values represent significance of IH or SH vs. Nox.