Figure 2
Promoter CpG methylation of DLEC1 in lymphoma cell lines. A. Diagram shows the DLEC1 CpG island, exon 1 (indicated with a rectangle), and CpG sites (short vertical lines). Numbers from 1 to 61 indicate the individual CpG sites. B. DLEC1 methylation as measured by MSP in lymphoma cell lines. M: methylated; U: unmethylated; DLBCL: diffuse large B-cell lymphoma; BL: Burkitt lymphoma; HL: Hodgkin lymphoma; NL: nasal NK/T-cell lymphoma. C. High resolution mapping of the methylation status of the DLEC1 promoter by BGS in normal PBMCs, and representative BL and HL cell lines. The DLEC1 transcription start site is shown as bent arrows. Circles, CpG sites analyzed; row of circles, an individual promoter allele that was cloned, randomly selected, and sequenced; filled circle, methylated CpG site; open circle, unmethylated CpG site.