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Table 1 Current Immunoscore procedure and reagents

From: Cancer classification using the Immunoscore: a worldwide task force

Procedure Current recommended steps
Tumor selection Block which is the most infiltrated by the immune cells and containing the core of the tumor (CT) and the invasive margin (IM)
Sample preparation 2 paraffin sections of 4-microns of the tumor block deposited in deionized water on Superfrost-plus slides
Immuno-histochemistry (IHC) 2 single stainings using IVD certified antibodies
Antigen retrieval CC1 tris-based buffer pH8
Primary antibody CD3 (2GV6, Ventana) and CD8 (C8/144, Dako)
Primary antibody diluant K 004 (Clinisciences) for CD8
Secondary reagents Ultraview TM DAB (Ventana)
Counterstaining Hematoxillin II (Ventana)
Autostrainer Benchmark XT (Ventana)
Scanner NanoZoomer 2.0-HT (Hammamatsu)
Digital pathology Architect XD software (Definiens)
Immunoscore quantification Immunoscore Plug-in (INSERM / AP-HP)