Figure 5

Effect of FoxM1 deletion on the expression of various cell cycle regulatory factors and MMP-2, MMP-9, and VEGF. A and B, real-time quantitative PCR and Western blot analysis showed that FoxM1 siRNA inhibited the expression of MMP-2, MMP-9, and VEGF at mRNA and protein levels in Caki-1 and 786-O cells. C, FoxM1 siRNA inhibited the activity of MMP-2 and MMP-9 based on gelatin zymography assay in Caki-1 and 786-O cells. D, FoxM1 siRNA inhibited the activity of VEGF in Caki-1 and 786-O cells. The experiments were repeated thrice. *, P < 0.05; **, P < 0.01, relative to control.