Phenotypes and clinical significance of circulating CD4+CD25+ regulatory T cells (Tregs) in patients with acute-on-chronic liver failure (ACLF)

Table 3 Representative amino acid sequences of monoclonal TCRBV families in CD4 ⁺ CD25 ⁺ Tregs from patients with ACLF or CHB

Patients^a	Vbeta		CDR3	BJ		Ratio
43 (ACLF)	BV20	SGFYLCAWS	TGTGHSPLH	FGNGTRLTVTED	1.6	17/21
70 (ACLF)	BV20	SGFYLCAWS	TGTGHSPLH	FGNGTRLTVTED	1.6	19/23
67 (ACLF)	BV20	SGFYLCAWS	TGTGHSPLH	FGNGTRLTVTED	1.6	13/22
6 (ACLF)	BV20	SGFYLCAWR	ISHTGEL	LFGEGSRLTVLED	2.2	16/22
16 (ACLF)	BV20	SGFYLCAWS	GGSNQPQ	HFGDGTRLSILEDL	1.5	13/20
51 (ACLF)	BV18	SAAYFCASS	RTGDTEA	FFGQGTRLTVVED	1.1	16/21
46 (ACLF)	BV13.1	SVYFCASS	EVRTAFYEQ	YFGPGTRLTVTED	2.7	14/21
25 (ACLF)	BV12	SVYFCAIS	DGQVVEQ	YFGPGTRLTVTED	2.7	15/22
47 (ACLF)	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	20/20
51 (ACLF)	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	20/20
10 (ACLF)	BV5.1	SALYLCASS	LEQLTRANKQ	NFGPGTRLTVLED	2.3	17/23
20 (CHB)	BV22	SAMYFCASK	GSREGLVVNEQ	FFGPGTRLTVLE	2.1	18/22
17 (CHB)	BV20	SGFYLCAWS	TGTGHSPL	HFGNGTRLTVTED	1.6	21/23
57 (CHB)	BV18	SAAYFCASS	DDQETQ	YFGPGTRLLVLED	2.5	21/21
45 (CHB)	BV17	TAFYLCASS	IVNGGQPQ	HFGDGTRLSILED	1.5	21/22
17 (CHB)	BV15	TALYFCATS	DPASGRTNEQ	FFGPGTRLTVLED	2.1	15/22
44 (CHB)	BV13.1	SVYFCASS	YSGQGIDGY	TFGSGTRLTVVED	1.2	16/22
3 (CHB)	BV11	SQYLCATT	SSGGVDTQ	YFGPGTRLTVLED	2.3	21/23

When the GMSP profile of a TCRBV family displayed a single peak, the corresponding PCR products re-amplified and the CDR3 were sequenced after cloning. The amino acid sequences of the max rate of TCRBV families are shown. ^aThe clinical types of patients were indicated in brackets.

ISSN: 1479-5876