Figure 1

Percentages of CD4⁺CD25⁺Tregs within total CD4⁺T population in peripheral blood in ACLF, CHB patients, and healthy controls. The CD4⁺CD25⁺ Tregs are gated from a mononuclear cell subset of peripheral whole blood. (A) Frequencies of CD4⁺CD25^high Tregs in peripheral blood from three groups (ACLF, CHB patients, and healthy controls, HC). Data are expressed as box plots, in which the middle of the black solid line is the median frequency of Tregs. Vertical lines represent the 10^th and 90^th percentiles. The p values of multiple comparisons were calculated using the Kruskal–Wallis H nonparametric test. (B) Representative scatter plots are obtained by flow cytometry using antibodies against CD4 and CD25. CD4⁺CD25⁺ Tregs were separated into CD4⁺CD25^high, CD4⁺CD25^low subsets, defined by the fluorescence intensity of CD25 (> 50) obtained using isotypic control antibody.