Figure 1

Immunofluorescent analysis of A2058 melanoma cells. A2058 cells were 100% positive for both MCSP (a) and MCAM (b). Less than 1% of cells were positive for both ABCB5 (c) and CD271 (d). All A2058 cells were CD45 negative (e). Anti-mouse A488-conjugate secondary antibody only staining was used as negative control and 100% of cells were negative (f). All images are an overlay of blue (DAPI) and green fluorescence (specific marker), with the same exposure for green fluorescence. Original magnification 400x. Scale bars, 100 μm.