Figure 1

Multilineage development of the human immune system in humanized mice. Samples were prepared for flow cytometry as described in the Materials and Methods. Dead cells and debris were excluded on FSC-A vs. SSC-A dot plot, doublets were excluded on FSC-A vs. FSC-H dot plots (not shown). (A) Multilineage development in bone marrow of a 12-week old humanized mouse. Both myeloid and lymphoid lineages were present. (B) Peripheral blood of a 12-week old “humanized” mouse. Mature forms of B cells (CD19+), CD4 and CD8 T cells, monocytes (CD14+) and neutrophils (CD15) can be easily identified. (C) Spleen of a 12-week old “humanized” mouse: CD4+ and CD8+ T cells, CD19 B cells, macrophages (CD19-CD68+) and NK cells (CD56+) are shown. (D) Immunohistochemical staining for human T cell markers, CD4 and CD8, B cell marker, CD20, and macrophage marker, CD68, in the spleen of 12-week old humanized mouse. Magnification 20x. Data are representative of >20 mice.