Figure 2
From: Gene silencing of IL-12 in dendritic cells inhibits autoimmune arthritis
Immune modulation by IL-12 shRNA silenced DC. (A) IL-12 silenced DC promote Th2 cytokine production. Control and siRNA-treated DC were cultured with allogeneic T cells for 3 days. Co-cultured T cells were harvested and gene expression of IFNγ, IL-2, IL-4 and IL-10 were detected by RT-PCR. (B) DC silenced with IL-12 shRNA inhibited allogeneic T cell proliferation. DC (1 × 105 cells/well) were co-cultured with allo-T cells (5 × 105 cells/well) in 96-well plate for 72 h. 1 μCi/well of 3H-labelled thymidine was added to the culture for the last 16 h of culture and proliferation was assessed by scintillation counting. Results were expressed as the mean counts per min of triplicate cultures ± SEM. * = P < 0.05.