Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury

Fouts, Derrick E; Pieper, Rembert; Szpakowski, Sebastian; Pohl, Hans; Knoblach, Susan; Suh, Moo-Jin; Huang, Shih-Ting; Ljungberg, Inger; Sprague, Bruce M; Lucas, Sarah K; Torralba, Manolito; Nelson, Karen E; Groah, Suzanne L

doi:10.1186/1479-5876-10-174

Table 3 Human and bacterial proteins potentially contributing directly or indirectly to host-pathogen interactions in the urinary tract

From: Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury

Human protein	Gene name	PSMs	Functional role	Urine donor
Protein S100-A9 Calprotectin L1H subunit	S10A9	60	Pro-inflammatory, metal ion-chelating	1,16,33,36,51
Protein S100-A8, Calprotectin L1L subunit	S10A8	10	Pro-inflammatory, metal ion-chelating	1,16,36,51
Protein S100-A12	S10AC	14	Pro-inflammatory	36
Myeloperoxidase	PERM	13	Microbicidal	36,51
Eosinophil peroxidase	PERE	4	Microbicidal	51
Lactotransferrin	TRFL	6	Pro-inflammatory, iron-chelating	36
14-3-3 protein sigma	SFN	5	DNA damage response, cell proliferation	16
SNC66 protein	-	12	Secreted, Ig-like domain	51
Heat shock protein beta-1	HSPB1	20	Anti-inflammatory, cell proliferation	1
Annexin A2	ANXA2	8	Cell proliferation, cell adhesion	16
Uromodulin	UMOD	78	Cell protection, inhibitor of Ca crystallization,	1,16,31,36,37,39,51
Cystatin-B	CYTB	8	Immunomodulatory, cathepsin inhibitor	1,16
14-3-3 protein zeta/delta	YWHAZ	2	Adaptor protein, tyrosine phosphorylation pathways	1,16
Serpin B3	SPB3	7	Immunomodulatory, serine protease inhibitor	16
Small proline-rich protein 3	SPRR3	7	Cell repair and proliferation	16
Annexin A1	ANXA1	28	Anti-apoptotic, T-cell differen-tiation, signaling pathways	1,16,31,34,37,39
Glutathione S-transferase P	GSTP1	3	Anti-apoptotic, tyrosine phosphorylation pathways	1,16,31,34,37
Bacterial protein	Species ^*
Colicin receptor CirA	Ec	2	Iron/colicin-binding	36
OM heme/hemoglobin receptor ChuA	Ec	12	Iron-binding	51
Putative pesticin receptor Psn	Ec	23	Iron-binding	51
Ferrienterobactin receptor FepA	Eh	9	Iron-binding	51
Putative fimbrillin MatB	Ec	2	Adhesion	51
Flagellin protein type B FliC	Pa	13	Mobility and adhesion	51
Flagellin protein FliC	Eh	8	Mobility and adhesion	51
Flagellin	Ec	7	Mobility and adhesion	51
Ferrienterobactin receptor FepA	Ec	22	Iron-binding	36, 51

Section one of the table lists proteins with potential pro/anti-inflammatory, immune-modulatory and microbicidal activities. Section two lists identified bacterial proteins implicated in virulence/survival or serving as a target for host defensive mechanisms.
PSMs: the highest number of peptide-spectral matches (PSMs) is provided for each listed protein * for species abbreviations, see Table 2 (minimal Mascot Percolator PEP value: 10^-4).

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ISSN: 1479-5876

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