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Table 3 Human and bacterial proteins potentially contributing directly or indirectly to host-pathogen interactions in the urinary tract

From: Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury

Human protein Gene name PSMs Functional role Urine donor
Protein S100-A9 Calprotectin L1H subunit S10A9 60 Pro-inflammatory, metal ion-chelating 1,16,33,36,51
Protein S100-A8, Calprotectin L1L subunit S10A8 10 Pro-inflammatory, metal ion-chelating 1,16,36,51
Protein S100-A12 S10AC 14 Pro-inflammatory 36
Myeloperoxidase PERM 13 Microbicidal 36,51
Eosinophil peroxidase PERE 4 Microbicidal 51
Lactotransferrin TRFL 6 Pro-inflammatory, iron-chelating 36
14-3-3 protein sigma SFN 5 DNA damage response, cell proliferation 16
SNC66 protein - 12 Secreted, Ig-like domain 51
Heat shock protein beta-1 HSPB1 20 Anti-inflammatory, cell proliferation 1
Annexin A2 ANXA2 8 Cell proliferation, cell adhesion 16
Uromodulin UMOD 78 Cell protection, inhibitor of Ca crystallization, 1,16,31,36,37,39,51
Cystatin-B CYTB 8 Immunomodulatory, cathepsin inhibitor 1,16
14-3-3 protein zeta/delta YWHAZ 2 Adaptor protein, tyrosine phosphorylation pathways 1,16
Serpin B3 SPB3 7 Immunomodulatory, serine protease inhibitor 16
Small proline-rich protein 3 SPRR3 7 Cell repair and proliferation 16
Annexin A1 ANXA1 28 Anti-apoptotic, T-cell differen-tiation, signaling pathways 1,16,31,34,37,39
Glutathione S-transferase P GSTP1 3 Anti-apoptotic, tyrosine phosphorylation pathways 1,16,31,34,37
Bacterial protein Species *    
Colicin receptor CirA Ec 2 Iron/colicin-binding 36
OM heme/hemoglobin receptor ChuA Ec 12 Iron-binding 51
Putative pesticin receptor Psn Ec 23 Iron-binding 51
Ferrienterobactin receptor FepA Eh 9 Iron-binding 51
Putative fimbrillin MatB Ec 2 Adhesion 51
Flagellin protein type B FliC Pa 13 Mobility and adhesion 51
Flagellin protein FliC Eh 8 Mobility and adhesion 51
Flagellin Ec 7 Mobility and adhesion 51
Ferrienterobactin receptor FepA Ec 22 Iron-binding 36, 51
  1. Section one of the table lists proteins with potential pro/anti-inflammatory, immune-modulatory and microbicidal activities. Section two lists identified bacterial proteins implicated in virulence/survival or serving as a target for host defensive mechanisms.
  2. PSMs: the highest number of peptide-spectral matches (PSMs) is provided for each listed protein * for species abbreviations, see Table 2 (minimal Mascot Percolator PEP value: 10-4).