Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury

Table 2 Bacterial profiles of urinary samples

Urine donor	Species (proteome)	Species (urine culture)	Species (16S rRNA profile)*	Leukocyte Esterase	WBC (no./hpf)
1(HC)	-	-	Lactobacillus	NEG	0
16(HC)	Lj	Lactobacillus spp	Lactobacillus	NEG	0-1
31(void)	Ec, Eh, Kp	-	Enterobacter, Enterococcus, Escherichia, Klebsiella, Lactobacillus, Streptococcus	TRA	1-2
33(void)	-	Ec, Ef	Enterobacter, Enterococcus, Escherichia, Klebsiella	2+	5-9
34(void)	Ec, Eh, Kp	Klebsiella oxytoca	Enterobacter, Escherichia, Lactobacillus, Streptococcus	2+	5-9
36(IC)	Ec, Eh	Ec	Enterobacter, Escherichia, Klebsiella, Lactobacillus, Streptococcus	NEG	0-1
37(IC)	-	-	Enterobacter, Enterococcus, Escherichia, Klebsiella, Lactobacillus, Pseudomonas, Streptococcus	NEG	1-2
39(IC)	Ec, Kp	Kp	Enterobacter, Klebsiella, Lactobacillus, Streptococcus	NEG	3-4
45(FC)	Eh, Kp, Pa, Pm^#	Ec, Ef, Kp, Pa, Ps	Enterobacter, Enterococcus, Escherichia, Klebsiella, Lactobacillus, Proteus, Pseudomonas, Streptococcus	NEG	0-1
51(FC)	Ec, Eh, Pa, Sp	Ec, Ef, Pa	Enterobacter, Enterococcus, Escherichia, Klebsiella, Pseudomonas, Streptococcus	2+	5-9

*Not comprehensive. 16S rDNA data is at the genus level.
^#The species for this sample were determined from urine cultures, but based on shotgun proteomic analysis after protein extraction from colonies.
Ec Escherichia coli, Eh Enterobacter hormaechei, Ef Enterococcus faecalis, Kp Klebsiella pneumoniae, Pa Pseudomonas aeruginosa, Pm Proteus mirabilis, Ps Providencia stuartii, Sp Streptococcus pneumoniae.
WBC = white blood cells, HC = healthy control, IC = intermittent catheter, FC = Foley catheter, NEG = negative, TRA = trace.

ISSN: 1479-5876