Skip to main content

Table 1 Comparison of primers and genomic coordinates used in this study for methylation-specific PCR in comparison to Herman et al.[7]

From: Characterization of CDKN2A(p16) methylation and impact in colorectal cancer: systematic analysis using pyrosequencing

  

Position/AB060808.1

Position / Start codon

Sequence

CDS p16/CDKN2A exon 1

192221- > 192072

0 - > 149

ATGGAGCCGG…..GCCGATCCAG

This study

F1

192197- > 192176

24 - > 45

ATGGAGCCTTCGGCTGACTGGC

 

R1_primer

192050- > 192033

171 - > 188

CCCGCCATCCCCTGCTCC

 

R2_primer

192077- > 192060

144 - > 161

CCCTCTACCCACCTGGAT

Herman

F1

192301- > 192278

−80 - > −57

TCACCAGAGGGTGGGGCGGACCGC

 

R1

192172- > 192151

49 - > 70

TTAACAAAAAAAAAAAACTAAACTCCTC