Figure 3

A) Mean percent-methylation ± standard deviation of each CpG site within the region analysed. The average methylation across all sites was 8.3% in normal mucosa and 17.6% in colorectal cancer. The percent-methylation at each CpG site is significantly (p < 0.0001, all) larger in the tumor compared with normal tissue. B) Distribution of methylation in normal tissues and tumor. 7.8% of all cases should a greater percentage of methylation in normal tissues than in tumor. Most cases showed slightly more methylation in tumor than normal while 20.6% of cases were considered methylation positive (at least 20% more methylation in tumor).