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Figure 2 | Journal of Translational Medicine

Figure 2

From: Characterization of CDKN2A(p16) methylation and impact in colorectal cancer: systematic analysis using pyrosequencing

Figure 2

A): Serial dilutions of 100% and 0% methylated DNA were combined and pyrosequencing used to analyse the measured and theoretical percentage of methylated DNA as a function of the number of PCR cycles. A strong correlation between the measured and theoretical percentage of methylation was observed, particularly for PCR cycle numbers of 35 or more. B) Influence on methylation background as a function of PCR cycle numbers. C) In contrast to pyrosequencing, only a poor correlation between the measured and theoretical percentage of methylation was observed using methylation-specific PCR (MSP) which also showed a considerable dependency on PCR cycle number. D) Comparison of measured and theoretical percentage of methylation for 40 and 50 PCR cycles obtained using pyrosequencing and MSP.

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