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Table 2 SVF intra-cellular proteome differences following culturing in the presence or absence of adipocytes

From: Analysis of in vitro secretion profiles from adipose-derived cell populations

UniProt accession No. Protein Name % Coverage (No. of peptides) SVF (+) : SVF (−) 115/116 SVF (+) : SVF (−) 114/117
Significant Up-Regulated Proteins
O60240 Perilipin-1 31 % (9) 2.73 2.44
P33121 Long-chain-fatty-acid--CoA ligase 1 19 % (10) 1.71 2.01
Q99541 Perilipin-2 46 % (15) 1.65 1.34
P23141 Liver carboxylesterase 1 42 % (20) 1.63 1.50
Q9BRX8 Uncharacterized protein C10orf58 31 % (8) 1.53  
Q16853 Membrane primary amine oxidase 51 % (52) 1.51 1.35
P21695 Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic 24 % (5) 1.46 1.52
Q12797 Aspartyl/asparaginyl beta-hydroxylase 31 % (17) 1.38 1.39
P05091 Aldehyde dehydrogenase, mitochondrial 43 % (19) 1.37 1.37
Q14315 Filamin-C 28 % (37) 1.29 1.56
Q9BXN1 Asporin 33 % (8) 1.29 1.29
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial 39 % (9) 1.29  
Q9UHG3 Prenylcysteine oxidase 1 30 % (11) 1.28  
P49327 Fatty acid synthase 20 % (29) 1.17 1.48
P42765 3-ketoacyl-CoA thiolase, mitochondrial 50 % (12) 1.17 1.29
P05120 Plasminogen activator inhibitor 2 57 % (24) 1.13 1.50
P09601 Heme oxygenase 1 53 % (15) 1.13 1.41
P15090 Fatty acid-binding protein, adipocyte 85 % (24) 0.86 0.54
P30740 Leukocyte elastase inhibitor 28 % (8) 0.81 0.70
P04179 Superoxide dismutase [Mn], mitochondrial 50 % (14) 0.77  
P29966 Myristoylated alanine-rich C-kinase substrate 42 % (9) 0.73  
P43490 Nicotinamide phosphoribosyltransferase 45 % (15) 0.68 0.66
P40261 Nicotinamide N-methyltransferase 22 % (4) 0.60 0.65
P20591 Interferon-induced GTP-binding protein Mx1 22 % (8) 0.59  
  1. Replicate SVF samples were cultured in the presence (+) or absence (−) of adipocytes for 72 hours in Standard Media. The SVF cells were collected, prepared and the tryptic peptides were labeled with an isobaric tag (114, 115, 116 or 117) for iTRAQ analysis using a Qstar Elite. A total of 2220 proteins were identified but only those which were significantly (p-value < 0.05) differentially expressed between the proteomes of the SVF cultured in the presence (+) or absence (−) of adipocytes have been included. For each of these proteins, the UniProt identification number, protein name, percentage of protein coverage (and number of unique peptides contributing to the sequence coverage), and fold change between the proteins in the SVF cultured with adipocytes versus the SVF cultured alone, for both replicates, are included.