Analysis of in vitro secretion profiles from adipose-derived cell populations

Table 2 SVF intra-cellular proteome differences following culturing in the presence or absence of adipocytes

UniProt accession No.	Protein Name	% Coverage (No. of peptides)	SVF (+) : SVF (−) 115/116	SVF (+) : SVF (−) 114/117
Significant Up-Regulated Proteins
O60240	Perilipin-1	31 % (9)	2.73	2.44
P33121	Long-chain-fatty-acid--CoA ligase 1	19 % (10)	1.71	2.01
Q99541	Perilipin-2	46 % (15)	1.65	1.34
P23141	Liver carboxylesterase 1	42 % (20)	1.63	1.50
Q9BRX8	Uncharacterized protein C10orf58	31 % (8)	1.53
Q16853	Membrane primary amine oxidase	51 % (52)	1.51	1.35
P21695	Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic	24 % (5)	1.46	1.52
Q12797	Aspartyl/asparaginyl beta-hydroxylase	31 % (17)	1.38	1.39
P05091	Aldehyde dehydrogenase, mitochondrial	43 % (19)	1.37	1.37
Q14315	Filamin-C	28 % (37)	1.29	1.56
Q9BXN1	Asporin	33 % (8)	1.29	1.29
Q16836	Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial	39 % (9)	1.29
Q9UHG3	Prenylcysteine oxidase 1	30 % (11)	1.28
P49327	Fatty acid synthase	20 % (29)	1.17	1.48
P42765	3-ketoacyl-CoA thiolase, mitochondrial	50 % (12)	1.17	1.29
P05120	Plasminogen activator inhibitor 2	57 % (24)	1.13	1.50
P09601	Heme oxygenase 1	53 % (15)	1.13	1.41
P15090	Fatty acid-binding protein, adipocyte	85 % (24)	0.86	0.54
P30740	Leukocyte elastase inhibitor	28 % (8)	0.81	0.70
P04179	Superoxide dismutase [Mn], mitochondrial	50 % (14)	0.77
P29966	Myristoylated alanine-rich C-kinase substrate	42 % (9)	0.73
P43490	Nicotinamide phosphoribosyltransferase	45 % (15)	0.68	0.66
P40261	Nicotinamide N-methyltransferase	22 % (4)	0.60	0.65
P20591	Interferon-induced GTP-binding protein Mx1	22 % (8)	0.59

Replicate SVF samples were cultured in the presence (+) or absence (−) of adipocytes for 72 hours in Standard Media. The SVF cells were collected, prepared and the tryptic peptides were labeled with an isobaric tag (114, 115, 116 or 117) for iTRAQ analysis using a Qstar Elite. A total of 2220 proteins were identified but only those which were significantly (p-value < 0.05) differentially expressed between the proteomes of the SVF cultured in the presence (+) or absence (−) of adipocytes have been included. For each of these proteins, the UniProt identification number, protein name, percentage of protein coverage (and number of unique peptides contributing to the sequence coverage), and fold change between the proteins in the SVF cultured with adipocytes versus the SVF cultured alone, for both replicates, are included.

ISSN: 1479-5876