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Table 4 T-cell responses detected in TIL infusion products

From: Adoptive cell therapy with autologous tumor infiltrating lymphocytes and low-dose Interleukin-2 in metastatic melanoma patients

a) IFN-γ ELIspot analyses
Patient ID 1 2 3 6 7 11
HLA-type A2 A2/A3 A3, A11 A2 A2 A1, A3
A2 Bcl-2WLD NR 43 - NR 301 -
Bcl-xRIA NR 165 - NR 197 -
hTERTILA 180 456 - 363 462 -
CB9L2ALY 232 135 - 293 441 -
CB9 204ILI 488 502 - 325 408 -
NY-ESO 1SLL 180 564 - 158 279 -
MAGE A1KVL NR 57 - NR 190 -
MAGE A3FLW 91 67 - 118 250 -
SUR1M2LML ND 96 - ND 349 -
SUR9ELT ND 62 - ND 274 -
MART-1ELA ND 324* - 193* NR -
gp100ITD ND 106 - ND ND -
A3 Bcl-xRIA - 284 111 - - 59
SUR18K10RIS - ND 112 - - NR
SUR53DLA - 96 ND - - 82
Rho C (nat)RAG - ND 114 - - NR
Rho C (mod)RLG - ND 103 - - NR
TAGRLS - ND ND - - 42*
Number of responses/total screened 5/15 14/24 4/9 6/15 9/15 3/13
Background 99 46 86 130a/92b 131 29
b) MHC multimer staining
Patient ID 1 2 c 3 6 7 11 d
HLA-type A2 A2, A3, B7 A3, A11 A2 A2 A1, A3
A2 MART-1ELA 0.013 0.07* - 1.10* 0.008 -
gp100YLE 0.010 NR - 10.0 NR -
gp100KTW 0.010 NR -   NR -
gp100ITD 0.002 NR - 0.018 NR -
GnT-VVLP-9 0.015 NR - NR NR -
A3 TAGRLS - NR NR - - 0.60*
Number of responses/total screened 5/146 1/162 0/14 3/146 1/146 1/21
  1. a) IFN-γ ELIspot analyses; Responses are defined as number of spots per 1 x 105 T cells, except for patient 7 (2.5 x 104 cells) and patient 11 (1.25 x 104 cells). Background level shown at the bottom of the table has been subtracted in the shown responses. a/b: two different assays have been performed wherefore two background values are present.
  2. b) MHC multimer staining; Antigen-specific T cells are given in percentage of CD8+ T cells. Examples of MHC multimer stainings can be seen in Additional file 3. cThe TILs analyzed were frozen two days after infusion. dThe TILs analyzed were frozen one day after infusion.
  3. Last row indicates the number of detected responses out of the total number of peptides screened. NR: no reactivity, ND: reactivity testing not done, -: not tested due to HLA-discrepancy.
  4. *Indicating responses found in both methods. nat: natural, mod: modified.