Immunohistochemical (IHC) (200X) staining of alpha-smooth muscle actin for quantification of small vessel (≤ 15.0 μm) in ischemic quadriceps at day 21 after CLI induction (n = 8). A to E) indicating IHC stain of number of small vessels (≤ 15 μm) (red arrows) in five groups (A = control, B = CLI only, C = CLI + cilostazol, D = CLI + clopidogrel, E = CLI + combined therapy). F) * vs. other groups with different symbols, p < 0.001. Statistical analysis by ANOVA followed by Bonferroni multiple comparison post hoc test. Scale bars in right lower corner represent 50 μm. HPF = high-power field (200x). CLI = critical limb ischemia; Cil = cilostazol; Clo = clopidogrel.