Figure 5
RT-PCR and Western blot analysis in PANC-1 cells. Samples were obtained from PANC-1 cells treated with 300 nM of P276-00 and/or 70 nM gemcitabine at various time points as indicated in the figure. (A) Electrophoretic analysis of BNIP3, p8 and COX-2 mRNA. (B) protein expression analysis of antiapoptotic protein COX-2 and proapoptotic protein BNIP-3. (C) Densitometric analysis of the COX-2 and BNIP3 bands using the software Image J.