Figure 2
Effect of P276-00 and gemcitabine used singly or in combination on survival of BxPC-3, AsPC-1, PANC-1, Capan-1 and MIA PACA-2 pancreatic cancer cell lines. The cells were treated as described under Materials and Method section. There was significantly higher growth inhibition of the cells treated with gemcitabine (24 h) followed by P276-00 at IC50 concentration (1.5, 1.5, 1.6, 0.7 and 0.6 μM for AsPC-1, BxPC-3, Capan-1, MIA PaCa-2 and PANC-1 respectively) for 72 h or 96 h compared to the cells treated with either agent alone. P276-00 treatment alone in BxPC-3, AsPC-1, PANC-1, Capan-1 and MIA PaCa-2 showed 78%, 48%, 38%, 69% and 47% survival respectively.