Dose-dependent changes of LRP1 protein levels in cardiomyocytes exposed to VLDL or LDL under normoxic or hypoxic conditions. HL-1 cardiomyocytes were submitted to normoxia or hypoxia for 24 hours either in absence or presence of increasing dose of VLDL (A) or LDL (B) for the last 12 hours. Representative Western blot analysis showing LRP1 bands. Unchanged levels of β-actin were shown as loading control and used to normalize LRP1 bands. Results were shown as mean ± SEM of three independent experiments performed in duplicate.*P < 0.05 versus normoxic cells. # P < 0.05 versus cells incubated in absence of lipoproteins.