Figure 6

Implanted MDSC stimulated angiogenesis and reduced myofibroblasts, without affecting PDE 5 expression, while concurrent sildenafil protected the endothelium but increased myofibroblasts and upregulated PDE5. (n = 7/group). Protein extracts were obtained from region #3 adjacent to the infarction area and subjected to western blot analysis.  A:  representative immunoblots (n = 8), indicating band sizes.  B:  PDE 5 assayed in MDSC cultures in duplicate.  C:  Densitometric values corrected by GAPDH. Statistical differences are stated for untreated versus basal, and MDSC, and MDSC + sildenafil versus untreated; *p < 0.05; **p < 0.01;***p < 0.005.