Figure 3
From: Increased microRNA-155 expression in the serum and peripheral monocytes in chronic HCV infection
Increased TNFα and TLR8 expression in peripheral monocytes of chronic HCV treatment naïve patients. A-C. Total RNA was isolated from monocytes of healthy volunteers treated or not (n = 6-8), and from chronic HCV treatment naïve patients stimulated or not (n = 9-10) with 100 ng/ml LPS for 6-8 h. Real time PCR was carried out using gene-specific primers for TNFα (A), TLR4 (B) and TLR8 (C) expression. 18 S was used as a normalization control and the non-parametric Mann–Whitney test was employed for statistical analysis.