Decreased miR-125b expression in peripheral monocytes of chronic HCV treatment naïve patients. A-B. Total RNA was isolated from monocytes of healthy volunteers (n = 6), chronic HCV treatment naïve patients (n = 12) and sustained responders (n = 5) with miRNeasy kit (Qiagen). MiR-125b and miR-146a levels were detected by TaqMan miRNA assay (Applied Biosystems), and RNU48 was used as a normalization control. The non-parametric Mann–Whitney test was employed for statistical analysis.