Induction of miR-155 in peripheral monocytes of chronic HCV treatment naïve patients. A. Total RNA was isolated from the monocytes of healthy volunteers (n = 6), chronic HCV treatment naïve patients (n = 12) and sustained responders (n = 5) with miRNeasy kit (Qiagen). B. Monocytes of healthy controls and chronic HCV treatment naïve patients were stimulated with 100 ng/ml LPS for 6-8 h. TaqMan miRNA assay (Applied Biosystems) was used for detection of miR-155. RNU48 was used as a normalization control. The non-parametric Mann–Whitney test was employed for statistical analysis.