Figure 3
From: Novel HER2 Aptamer Selectively Delivers Cytotoxic Drug to HER2-positive Breast Cancer Cells in Vitro
Quantitative assay of HB5’s binding affinity. Beads coated with HER2 peptide (A) or HER2 ECD protein ( B ) were incubated with varying concentrations of FITC-labeled aptamers. The mean fluorescence intensity of the unselected DNA library (background binding) at each concentration was subtracted from the mean fluorescence intensity of the corresponding aptamer. The actual fluorescence intensity was fitted into Sigmaplot to determine the apparent Kd.