Frequencies of peripheral regulatory B cells of different study groups. Frequencies of peripheral regulatory B cells were analysed in the healthy donor (Healthy), patients with chronic hepatitis B infection (CHB), and patients with hepatocellular carcinoma (HCC) before surgery (HCCb), 1-2 days (HCCa1) or about 7 days (HCCa7) after surgery. Representative flow cytometry analysis of peripheral IL10+CD19+ cells were measured and presented (A). Frequency of IL10+CD19+ cells of total CD19+ B cells in the peripheral blood (B). The mean ± SEM is shown, *, **and *** stand for P < 0.05, 0.01 and 0.001 respectively.