RT-PCR and Western blot analysis of HMGB1 expression in normal (N), para-tumor (P) and tumor tissue (T) of all 11 HCC cases fresh tissues. (a) Representative RT-PCR results of two cases, and (c) Representative Western blot results of two cases. (b) Relative quantity of HMGB1 mRNA expression, and (d) protein expression. Each value which represents the average of 11 cases expressed as the mean ± SE. Human GAPDH served as an internal control, the gray-scale comparison between HMGB1 and GAPDH was used to quantify the bands of RT-PCR and western blot results. * p < 0.001, compare to normal (N) and para-tumor (P).