Figure 1

Histological resemblance between patient tumor and resulting tumorgraft following sequential grafting into immunocompromised mice. Representative sections stained with hematoxylin and eosin of indicated tumor samples (Scale bars indicate 100 μm). A) Adenocarcinoma of pancreas with desmoplastic stromal reaction in original patient tumor. 1^st generation tumorgraft (total time in vivo, 217 days) with preservation of atypical glandular structures with inspissated mucinous material surrounded by fibrous stroma. 2^nd generation tumorgraft (303 days) with similar histology. B) Adenocarcinoma of colon with numerous atypical glands containing mucinous material and necrotic debris with surrounding fibrovascular connective tissue. 1^st generation tumorgraft after 101 days reveals dilated atypical ducts with mucin and necrotic debris in lumens comprised of multiple columnar layers of atypical epithelium surrounded by fibrovascular stroma. 2^nd generation graft after 177 days with similar histology. C) Lung carcinoma with prominent fibrovascular connective tissue in original patient neoplasm that becomes progressively overgrown by malignant cells in 1^st (216 days) and 2^nd (286 days) generation tumor grafts. D) Cervical carcinoma with fibromucinous stroma in original patient neoplasm, with expansion of large islands of malignant cells surrounded by fibrovascular stroma in 1^st (282 days) and 2^nd (345 days) generation tumorgrafts.