Figure 3From: Transient and intensive pharmacological immunosuppression fails to improve AAV-based liver gene transfer in non-human primatesTransient pharmacological immunosuppression fails to enhance rAAV5-mediated gene transfer to the liver in macaques. A) Sequential follow-up of PBGD activity in homogenates from liver samples taken at the indicated time points. Normal range was defined as means ± 2 standard deviation of a separate group of four non-injected female macaques. B) Analyses of human PBGD mRNA content by quantitative RT-PCR in liver homogenates from necropsies taken at sacrifice (week 25). C) Immunohistochemistry detection of hepatocytes overexpressing PBGD on formalin-fixed and paraffin-embedded sections from liver biopsies obtained from the indicated animals at sacrifice. Data corresponding to necropsy samples (week 25) are expressed as median ± standard deviation of eight different lobes in each macaque.Back to article page