Figure 1
An immunosuppressive pharmacological regimen delays and weakens immunity against rAAV5 capsid antigens. A) Schematic timeline representation of the administration of two rAAV serotype 5 vectors in macaques under combined pharmacological immunosuppression. rAAV5-PBGD was injected first and rAAV5-egfp second. Animals are colour coded, identified by the NHP-digit code and their timeline represented by a horizontal arrow. B) Sequential follow-up of serum rAAV5 neutralizing antibodies in the serum of the colour-coded animals. C) In vitro mitogenic response of peripheral blood mononuclear cells (PBMC) at the indicated time points to rAAV5 capsids, measured by 3 H-Thy incorporation. D) Sequential follow-up of proviral rAAV5-PBGD DNA detection from serum samples of the colour-coded animals by quantitative PCR of vector DNA. Each dot represents the average of two independent measurements for each sample at a time point.