Figure 2
From: Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery
SDS-PAGE analysis of B. mori silk fibroins prepared with various solutions. Silk fibroins were prepared from four different calcium-alcohol solutions (as described below) then dissolved in hot water. The range of molecular weight of the proteins produced by each solution was determined by SDS-PAGE with 12% acrylamide gel and 4% condensing gel, which was stained with 0.25% Coomassie Brilliant Blue R-250. Lanes: M, marker. (A) silk fibroin prepared from Ca(NO3)2·4H2O-methanol solution. (B) silk fibroin prepared from Ca(NO3)2·4H2O-ethanol solution. (C) silk fibroin prepared from CaCl2-methanol-H2O solution. (D) silk fibroin prepared from CaCl2-ethanol-H2O solution. (E) Degummed silk fibroin.