Figure 1From: Coculture with hematopoietic stem cells protects cardiomyocytes against apoptosis via paracrine activation of AKTshows the experimental setup. After 48 h of coculture with NRVCM two cell sorting steps generated a highly purified population of conditioned murine hematopoietic SC that were used for RNA isolation and microarray analysis. Conditioned NRVCM were labeled with Annexin V and tested for apoptosis by FACS. Finally, coculture supernatants were concentrated and used for ELISA and stimulation of NRVCM.Back to article page