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Figure 6 | Journal of Translational Medicine

Figure 6

From: Validation of analytical methods in GMP: the disposable Fast Read 102® device, an alternative practical approach for cell counting

Figure 6

Linearity and range on wPB and wBM. Linearity and range was evaluated by Fast Read 102 ® device, as above described. The assay was performed on wPB (A) and wBM (B). The Operators tested different dilutions of cell suspension with Tuerk solution (undiluted, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128) in order to verify the best range of dilution to use. To avoid any potential interference of the Tuerk lysing solution and the Trypan Blue vital dye, in this case, the test only considers the total cell count and not cell viability. The regression line was calculated using the average of values obtained from Op1 and Op2’s counts at each dilution. The best range of dilution, in order to obtain a slope line value very similar to 1, is between 1:8 and 1:128. a1) wPB dilution range: 1:4, 1:8, 1:16, 1:32, 1:64, 1:128; a2) wPB dilution range: 1:8, 1:16, 1:32, 1:64, 1:128; b1) wBM dilution range: 1:4, 1:8, 1:16, 1:32, 1:64, 1:128; b2) wBM dilution range: 1:8, 1:16, 1:32, 1:64, 1:128. Op1 = operator 1, Op2 = operator 2.

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