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Figure 5 | Journal of Translational Medicine

Figure 5

From: Validation of analytical methods in GMP: the disposable Fast Read 102® device, an alternative practical approach for cell counting

Figure 5

Precision and Repeatability on MSCs. As previously reported, also for MSCs, the assay was performed only using the Fast Read 102® chamber on MSCs. Operators 1 and 2 tested their cell counts three times. Cell viability was evaluated using Trypan Blue vital dye with a 1:2 dilution. To obtain the intra operator CV%, each operator calculated the average and SD of their three counts (a: Op1 raw data; b: Op2 raw data). The inter operator CV% viability was then calculated (c). CV = coefficient of variation, MSC = mesenchymal stem cells, Op1 = operator 1, Op2 = operator 2.

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