Skip to main content
Figure 4 | Journal of Translational Medicine

Figure 4

From: Validation of analytical methods in GMP: the disposable Fast Read 102® device, an alternative practical approach for cell counting

Figure 4

Precision and Repeatability on MNCs. On the basis of accuracy data, the following experiments were made to test precision, repeatability, linearity and range only by using Fast Read 102®. The assay was performed only using the Fast Read 102® chamber on MNCs. Operators 1 and 2 tested their cell counts three times. Cell viability was evaluated using Trypan Blue vital dye with a 1:2 dilution. To obtain the intra operator CV%, each operator calculated the average and SD of their three counts (a): Op1 raw data; (b): Op2 raw data. The inter operator CV% viability was then calculated (c). CV = coefficient of variation, MNC = mononuclear cells, Op1 = operator 1, Op2 = operator 2.

Back to article page