Figure 4From: Validation of analytical methods in GMP: the disposable Fast Read 102® device, an alternative practical approach for cell countingPrecision and Repeatability on MNCs. On the basis of accuracy data, the following experiments were made to test precision, repeatability, linearity and range only by using Fast Read 102®. The assay was performed only using the Fast Read 102® chamber on MNCs. Operators 1 and 2 tested their cell counts three times. Cell viability was evaluated using Trypan Blue vital dye with a 1:2 dilution. To obtain the intra operator CV%, each operator calculated the average and SD of their three counts (a): Op1 raw data; (b): Op2 raw data. The inter operator CV% viability was then calculated (c). CV = coefficient of variation, MNC = mononuclear cells, Op1 = operator 1, Op2 = operator 2.Back to article page