Table 1 Nucleotide sequences of 10 primers for qRT-PCR.
Gene | Primer | Sequence (5' - 3') | Melting temperature (°C) | Product size (bp) |
|---|---|---|---|---|
β-actin | Forward | 5'-GTGGAGTGCCCAAGCACCA- 3' | 52 | 213 |
| Â | Reversed | 5'-CTCTAATGTCACGACGATTTC-3' | Â | Â |
Adiponectin | Forward | 5'-AGGAATTCTACTGCCGTCGA-3' | 55 | 179 |
| Â | Reversed | 5'-TCAAGGCGAGCTCGTATTTGA-3 | Â | Â |
AIP | Forward | 5'-GCTAGCAAACCACCTAAGTA-3 | 54 | 207 |
| Â | Reversed | 5'-GTGGTCCAGAACATAGTAGA-3 | Â | Â |
Zfp467 | Forward | 5'-CACGTGACACCTACCAAGTA-3 | 55 | 186 |
| Â | Reversed | 5'-CCGTAACAGGTATGAGTAGA-3 | Â | Â |
OCN | Forward | 5'-GCCCAGCTGTAACCACGATA-3 | 56 | 126 |
| Â | Reversed | 5'-CAGGTCCATAAGGTAGTAGA-3 | Â | Â |
OPN | Forward | 5'-GCATGCTACACCAACCAGTA-3 | 52 | 198 |
| Â | Reversed | 5'-CAGAAGTGGTGTACAGTAGA-3 | Â | Â |
COL I | Forward | 5'-GCAGCCCCATAGACGCCGTA-3 | 51 | 171 |
| Â | Reversed | 5'-CAGAGGTCATCCACCAATAGA-3 | Â | Â |
BSP | Forward | 5'-GCAGAGTAGTACTGCAGGTA-3 | 55 | 190 |
| Â | Reversed | 5'-CAGAGGTGTCCATAAGTAGA-3 | Â | Â |
LPL | Forward | 5'-GCGAGTGTAGCTCAGCCGTA-3 | 53 | 185 |
| Â | Reversed | 5'-CAGGTCCATAGAGGTAGTAGA-3 | Â | Â |
PPARγ | Forward | 5'-GTAGAGTGGCACAGTGGGTA-3' | 52 | 313 |
| Â | Reversed | 5'-TTACTTGCCATCAAGATTGTC-3' | Â | Â |