Figure 4

The mRNA and protein levels of Zfp467 in cultured adipocyte, osteoblast and undifferentiated ADSCs. The mRNA and protein levels of Zfp467 in adipocyte and osteoblast derived from ADSCs at day 14 and undifferentiated ADSCs (Day 0) were measured using qRT-PCR (A) and Western blot analysis (B). The data are representative of three independent experiments. The two si-Zfp467 vector (pSilencer4.1-si-Zfp467 with neomycin resistance), which produces specific siRNA (Zfp467-KD1 and -KD2) were used in the experiment. Scrambled siRNA was used as a control for siRNA-Zfp467. The efficiencies of Zfp467 RNAi in ADSCs were evaluated using qRT-PCR (C) and Western blot analysis (D).