Figure 2From: Suppression of zinc finger protein 467 alleviates osteoporosis through promoting differentiation of adipose derived stem cells to osteoblastsAdipocyte and osteoblast differentiation of cultured ADSCs. Microphotographs of ADSCs differentiated into adipocytes (A) and osteoblasts (D) at day 14. Bar scale = 50 μm. Differentiation in cultured ADSCs was monitored including oil red O staining of lipid droplets in terminal adipocyte differentiation (B) and von Kossa staining of calcium deposition in the extracellular matrix in terminal osteoblast differentiation (E). The qRT-PCR analysis of mRNA levels of specific adipogenic (adiponectin) (C) and osteogenic (alkaline phosphatase) markers (F) at day 7 and day 14 of differentiation as compared to day 0. The data are representative of three independent experiments.Back to article page