Figure 6

The percentage of each background peak over sequence-specific signal is calculated by dividing each background peak height with the average peak height of the proximal upstream and downstream sequence-specific peak height. Top panel depicts a trend line generated from all the "A" peak heights of the pyrogram shown in Figure 5. Middle panel depicts a trend line generated from all the "G, C and T" peak heights of the pyrogram shown in Figure 5. R² are shown on the upper right corners of both panels. Bottom depicts theoretical and extrapolated peak heights generated from the NDO and peak heights shown in top panel. The formula that is used is: Extrapolated peak= [Split Height + (Slope × Disp#)] × Nuc#. Average background is the average of all background signals. MAX background is the highest background signal over sequence specific signal. MIN background is the lowest background signal over sequence specific signal.