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Figure 5 | Journal of Translational Medicine

Figure 5

From: Pyrosequencing™ : A one-step method for high resolution HLA typing

Figure 5

Linear signal generation through a 72 nucleotide dispensation Pyrosequencing run. Shown on the top in red is the Pyrosequencing primer sequence from 5' end to 3' end in the direction the red arrow points to. In this Pyrosequencing reaction, nucleotide incorporation into DRB1*1201, 1302 starts immediately downstream of the 3' end of Pyrosequencing primer and ends at the 3' end template sequence shown. The polymorphic positions are shown and underlined in blue. Pyrogram is shown below template sequence. Y-axis represents peak heights. X-axis represents NDO.

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