Figure 4

The same NDO can generate unique pyrogram from DRB1*030101, 130101 Vs. DRB1*0319, 1320. Top pyrogram is generated from Pyrosequencing reaction in one well of a 96 well plate. Bottom pyrogram is generated from Pyrosequencing reaction in a separate well of a 96 well plate. The X axis of each pyrogram, from left to right, indicates the order of reagent addition. E represents enzyme. S represents substrate. The expected number of nucleotides incorporation into each pair of heterozygous DNA template and actual peak height are indicated for the normalizer peaks and differential peaks below each Pyrogram. At the bottom of the figure is shown expected ratio of peak heights and the ratio of normalized peak heights (in shadowed area).