Figure 2

The same NDO can generate unique pyrogram from DRB1*11011, 13011 and DRB1*1111, 1306. The two heterozygous templates used are DRB1*11011, 13011 and DRB1*1111, 1306. The SBT ambiguous positions are at 5'-298-299-3'. The Pyrosequencing primer is high lighted in purple color with its 3' end located at 5'-285-3'. Nucleotide incorporation starts at position 5'-286-3'. NDO is indicated on the left, from top to bottom. The nucleotides incorporated into each allele of template DRB1*11011, 13011 and each allele of template DRB1*11011, 13011 are highlighted in yellow at each nucleotide dispensation step except for the nucleotides involve in the ambiguities that are with the color red for , green for and black for .