Figure 1From: Pyrosequencing™ : A one-step method for high resolution HLA typingNDO optimization. Sequences of DRB1*11011, 13011 and DRB1*1111, 1306 are shown on the top (5'-286-305-3'). NDO 1 to NDO 5 are designed to pyrosequence these two pairs of heterozygous templates. X-axis represents the NDO. G, A, T and C represent the dNTP that is dispensed at each. Alphabetical numbers represent the dispensation step. E.g. the first step dispenses dATP. Y-axis represents theoretical peak height, shown as number of nucleotide incorporated into the two molecules of template alleles at each nucleotide dispensation step. Black peaks represent signals generated from DRB1*11011, 13011. Red peaks represent signals generated from DRB1*1111, 1306.Back to article page