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Figure 7 | Journal of Translational Medicine

Figure 7

From: Interferon-γ Added During Bacillus Calmette-Guerin Induced Dendritic Cell Maturation Stimulates Potent Th1 Immune Responses

Figure 7

T cell stimulation by DC matured with BCG alone or in combination with IFN-γ is mediated via identical co-stimulatory molecules DC were treated with 4.1 × 105 CFU/ml of BCG alone, BCG plus 1000 U/ml IFN-γ or left untreated (immature) for 24 h. The DC were harvested, washed, and placed in mixed leukocyte culture with allogeneic T cells at 1:100 ratio in triplicate wells of a microculture plate. Blocking antibodies were added to the DC at 1 μg/well for 1 h prior to the addition of T cells. Five days later culture supernatants collected for evaluating IFN-γ secretion by ELISA (B). The cells were pulsed with 3H-thymidine for an additional 24 h cells before harvest and quantification of incorporated radioactivity in a scintillation counter (A). Results shown in each panel are from one of two identical experiments.

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