|
Cell line
|
Ki67 (%)
|
Arginase II RT-PCR
|
Arginase activity (μg urea/mg protein) – control
|
Arginase activity (μg urea/mg protein) – rIFN-γ-treated cells
|
Cytostasis (%)
|
|---|
|
2774*
|
95
|
+
|
1.79
|
0.35
|
47.2 + 11.9
|
|
CAOV3
|
93
|
+
|
1.00
|
0.51
|
80.9 + 6.2
|
|
NMP-1
|
84
|
+
|
0.36
|
0.09
|
83.6 + 6.9
|
|
2008*
|
31
|
+
|
0.69
|
4.90
|
70.8 + 13.8
|
|
2008.C13
|
34
|
+
|
1.00
|
3.02
|
54.2 + 15.4
|
|
HEY
|
91
|
+/-
|
0.14
|
0.14
|
57.8 + 22.6
|
|
SKOV3
|
72
|
+/-
|
0.18
|
0.18
|
7.5 + 4.1
|
|
OVCAR3
|
54
|
+/-
|
0.08
|
0.09
|
44.1 + 19.1
|
- The percentage of Ki-67 was determined by immunocytochemistry, and the expression of arginase II was examined by RT-PCR as described in Materials and Methods. Arginase activity and growth inhibition were determined after 72 hours of incubation with control medium or rIFN-γ and are representative of 4 independent experiments. rIFN-γ was added daily to the cells. *Platinum-sensitive.
