rIFN-γ-mediated growth suppression of platinum-sensitive and -resistant ovarian tumor cell lines not dependent upon arginase inhibition

Table 1 Arginase activity in EOC cell lines

Cell line	Ki67 (%)	Arginase II RT-PCR	Arginase activity (μg urea/mg protein) – control	Arginase activity (μg urea/mg protein) – rIFN-γ-treated cells	Cytostasis (%)
2774*	95	+	1.79	0.35	47.2 + 11.9
CAOV3	93	+	1.00	0.51	80.9 + 6.2
NMP-1	84	+	0.36	0.09	83.6 + 6.9
2008*	31	+	0.69	4.90	70.8 + 13.8
2008.C13	34	+	1.00	3.02	54.2 + 15.4
HEY	91	+/-	0.14	0.14	57.8 + 22.6
SKOV3	72	+/-	0.18	0.18	7.5 + 4.1
OVCAR3	54	+/-	0.08	0.09	44.1 + 19.1

The percentage of Ki-67 was determined by immunocytochemistry, and the expression of arginase II was examined by RT-PCR as described in Materials and Methods. Arginase activity and growth inhibition were determined after 72 hours of incubation with control medium or rIFN-γ and are representative of 4 independent experiments. rIFN-γ was added daily to the cells. *Platinum-sensitive.

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