Figure 4From: The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicityCorrelation of GrB and IFN-γ secretion in the ELISPOT assay with cytotoxicity in 51Cr-release assay. Human α-FMP-CTL (7 day culture) were used as effector cells. Target cells were C1R.A2 pulsed with FMP, 5 × 103 cells per well. Incubation time was 4 h at 37°C for all three assays. At the 10:1 effector:target ratio the spots in the IFN-γ ELISPOT assay were too numerous to count. Background (CTL alone) was subtracted from the results. Data is representative of 3 experiments.Back to article page